Journal: Journal of Oral Microbiology
Article Title: Characterisation of novel Fusobacterium nucleatum bacteriophages and their efficacy in disrupting pathogenic dual-species biofilms
doi: 10.1080/20002297.2025.2584952
Figure Lengend Snippet: Location of protospacers targeted by F. nucleatum strain 2256 CRISPR system, that are found in the bacteriophage FNU4. The putative genes are represented by packaging (black), hypothetical (grey), head and capsid (green), lysis (red), tail (blue), transport and metabolism (yellow), and DNA manipulation (pink) (A). Genome maps of draft genomes of two of the F. nucleatum hosts for bacteriophages. In both F. nucleatum strain 2256 (B) and Fnp ATCC 10953 (C), the genome maps consist of tracks that are labeled from outside to center as track 1: prophage in dark green; tracks 2 & 3 mobile genetic elements including sites for replication (red), integration or excision (light green), defence-related genes (purple) and other transfer genes (olive green). Track 4 indicates the GC content graph, while the size of the genome is indicated by the labels on the innermost track. The bacteriophage FNU4 has homology to the region from 0.4 Mbp to 0.8 Mbp in the F. nucleatum strain 2256 map. Kernel density estimation (KDE) plots showing the uneven clustering of defence system loci across the genomes of F. nucleatum strains 2256 (D) and Fnp ATCC 10953 (E).
Article Snippet: The purified bacteriophage particles were visualised using a JEOL JEM−2100 transmission electron microscope (TEM) with 400-mesh carbon-coated copper grids (ProSciTech, Australia).
Techniques: CRISPR, Lysis, Labeling